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pegfp n1 fus tls flagc  (Addgene inc)


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    Addgene inc pegfp n1 fus tls flagc
    Pegfp N1 Fus Tls Flagc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pegfp n1 fus tls flagc/product/Addgene inc
    Average 93 stars, based on 9 article reviews
    pegfp n1 fus tls flagc - by Bioz Stars, 2026-03
    93/100 stars

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    pegfp n1 fus tls flagc 60362  (Addgene inc)
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    Photoactivation of <t>FUS</t> condensation. (A) Scheme of full-length FUS protein (top) and chimeric constructs used in this study <t>(bottom)</t> <t>(IDR,</t> intrinsically disordered region; RRM and RGG, RNA-binding motives; Zn, Zn-finger). (B) Cartoon of the imaging assay in HEK cells. (C) Representative images (z-projections) before (left) and after (right) photoactivation of 1 min at 0.02 mW 488 nm-laser power at the output. Scale bar, 10 μm. (D) Line profiles of highlighted regions in (C) (dotted line, before activation; full line, after activation).
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    Photoactivation of FUS condensation. (A) Scheme of full-length FUS protein (top) and chimeric constructs used in this study (bottom) (IDR, intrinsically disordered region; RRM and RGG, RNA-binding motives; Zn, Zn-finger). (B) Cartoon of the imaging assay in HEK cells. (C) Representative images (z-projections) before (left) and after (right) photoactivation of 1 min at 0.02 mW 488 nm-laser power at the output. Scale bar, 10 μm. (D) Line profiles of highlighted regions in (C) (dotted line, before activation; full line, after activation).

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Aberrant Phase Separation of FUS Leads to Lysosome Sequestering and Acidification

    doi: 10.3389/fcell.2021.716919

    Figure Lengend Snippet: Photoactivation of FUS condensation. (A) Scheme of full-length FUS protein (top) and chimeric constructs used in this study (bottom) (IDR, intrinsically disordered region; RRM and RGG, RNA-binding motives; Zn, Zn-finger). (B) Cartoon of the imaging assay in HEK cells. (C) Representative images (z-projections) before (left) and after (right) photoactivation of 1 min at 0.02 mW 488 nm-laser power at the output. Scale bar, 10 μm. (D) Line profiles of highlighted regions in (C) (dotted line, before activation; full line, after activation).

    Article Snippet: The DNA fragment encoding the IDR of human FUS (residues 1-215, extracted from Addgene plasmid #60362) was inserted by PCR-based cloning into the pCry2-mCh vector using Xho I- Kpn I restriction sites ( ).

    Techniques: Construct, RNA Binding Assay, Imaging, Activation Assay